What Is a Pipette?
A pipette is a laboratory tool used to transport a measured volume of liquid. It ensures accuracy and precision in scientific experiments. Pipettes are common in chemistry, biology, and medical labs.
Types of Pipettes
Micropipette
Micropipettes are used for very small liquid volumes, typically in microliters. These are ideal for molecular biology and microbiology tasks.
Volumetric Pipette
Volumetric pipettes offer high accuracy. They are designed to deliver one specific volume with precision.
Graduated Pipette
Graduated pipettes are marked with volume graduations. They allow for measuring different volumes, offering flexibility.
Pasteur Pipette
These are disposable pipettes, typically made from glass or plastic. They are used for transferring drops of liquid without measuring.
How to Use a Pipette Correctly
Using a pipette requires attention to technique.
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Choose the correct pipette for the volume.
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Attach the right tip.
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Pre-wet the tip to ensure accuracy.
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Press the plunger to the first stop.
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Immerse the tip slightly into the liquid.
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Slowly release the plunger to draw the liquid.
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Move to the target container.
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Press the plunger to the second stop to dispense.
Avoid bubbles. Always use a smooth motion.
Importance of Pipette Calibration
Pipette calibration ensures measurement accuracy. Over time, pipettes can become inaccurate due to wear. Regular calibration prevents errors and maintains consistency in results.
Signs Your Pipette Needs Calibration
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Volume inconsistency
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Leaking tips
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Resistance during plunger use
Annual calibration is recommended. Some labs calibrate more often, depending on usage.
Pipette Maintenance Tips
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Clean pipettes regularly
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Store them upright
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Avoid laying them flat with liquid inside
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Use proper tips only
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Handle with care
Routine maintenance prolongs pipette life and ensures accuracy.
Common Mistakes to Avoid
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Using the wrong pipette for the volume
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Not pre-wetting the tip
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Pipetting too fast
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Immersing tip too deep
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Skipping calibration checks
These mistakes reduce reliability. Always follow standard protocols.
Choosing the Right Pipette
Select based on:
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Volume range
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Frequency of use
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Required precision
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Compatibility with tips
Investing in quality pays off in the long run.
Pipette Safety Guidelines
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Wear gloves
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Use pipette aids for glass pipettes
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Never mouth pipette
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Dispose of tips properly
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Report damaged devices immediately
Lab safety must always come first.
Conclusion
Pipettes are essential for precision in scientific labs. By using them correctly, calibrating regularly, and maintaining proper hygiene, you ensure dependable results every time. Mastering pipette use boosts lab productivity and accuracy.
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